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https://doi.org/10.3938/NPSM.68.486
Three-Dimensional Structural Analysis of a Mouse Eyeball by Using Synchrotron Micro-CT
New Phys.: Sae Mulli 2018; 68: 486~490
Published online April 30, 2018;  https://doi.org/10.3938/NPSM.68.486
© 2018 New Physics: Sae Mulli.

Sung-Mi HAN1, Ki-Hong KIM*2, Se-Hee LEE2, Hong-Tae KIM3

1 Optical Convergence Technology Center, Catholic University of Daegu, Kyungsan 38430, Korea
2 Department Optometry & Vision Science, Catholic University of Daegu, Kyungsan 38430, Korea
3 Department of Anatomy, School of Medicine, Catholic University of Daegu, Daegu 42472, Korea
Correspondence to: kkh2337@cu.ac.kr
Received December 15, 2017; Revised April 9, 2018; Accepted April 13, 2018.
cc This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Three-dimensional (3D) histology is an imaging system for acquiring 3D structural information on cells or tissues. Understanding the 3D structure of tissues and cells by using 2D sections is difficult. A general 3D reconstruction method by stacking serial sectioned images of a tissue is hard work and can lead to reconstruction errors through sections that are torn or missing. Experiments were performed at the beamline 6C of the Pohang Light Source (PLS) using phase-contrast X-ray imaging method, a computer-controlled precision stage, and rotations in 0.18$^\circ$ ~ 0.2$^\circ$ increments through 180$^\circ$. Reconstructed images were compared with images from histology slides obtained from the same samples. In this study, a new 3D histology technique using synchrotron micro tomography was established and applied to the analysis of the 3D structure of a mouse eyeball.
PACS numbers: 42.30.-d
Keywords: Synchrotron radiation, X-ray micro-CT, Eyeball


April 2018, 68 (4)
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