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SERS-Based Sandwich Immunoassay for Alzheimer’s Disease Biomarker Detection Using Antibody Conjugated Magnetic Nanoparticles
New Phys.: Sae Mulli 2019; 69: 690~694
Published online July 31, 2019;
© 2019 New Physics: Sae Mulli.

1Seong Jin BACK, Gyeong Bok JUNG*
2Yeron LEE, Jeunghee PARK

1Department of Physics Education, Chosun University, Gwangju 61452, Korea
2Department of Advanced Materials Chemistry, Korea University Sejong Campus, Sejong 30019, Korea
Correspondence to:
Received April 2, 2019; Revised May 25, 2019; Accepted May 28, 2019.
cc This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Surface-enhanced Raman scattering (SERS)-based immunoassays have been developed for fast and ultrasensitive detection of biomarkers. In this study, we report a SERS-based sandwich immunoassay for the detection of the Alzheimer’s disease biomarker amyloid$\beta$(A$\beta$). A method combining magnetic separation and SERS was developed to detection of A$\beta$. Gold-coated magnetic nanoparticles (Fe$_3$O$_4$@Au MNPs) were prepared by immobilizing anti-A$\beta$ antibodies and was used in the separation and the concentration of the A$\beta$. Gold nanoparticles (Au NPs) were modified with detection antibodies, and antibody-conjugated Au NPs were labeled with 3, 3, diethylthia-tricarbocyanine iodide (DTTC), as the SERS probes. The correlation between the A$\beta$ concentration and the SERS signal was found to be linear within the range from 1 fM to 1 $\mu$M. As a result, we were able to detect A$\beta$ with a much lower limit of 1 fM. This technique allows for straightforward SERS-based bioassays for the quantitative detection of biomarkers and has potential for clinical applications.
PACS numbers: 74.25.nd, 42.62.Be, 87.15.M$-$, 42.15. Eq, 81.05.$-$t
Keywords:  Surface enhanced roman scattering (SERS), Alzheimer, Amyloid-$\beta$ (A$\beta$), Biomarker, Nanoparticle

October 2019, 69 (10)
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