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Review of the Principles and the Applications of Fluorescence Correlation Spectroscopy
New Phys.: Sae Mulli 2019; 69: 846~854
Published online August 30, 2019;
© 2019 New Physics: Sae Mulli.

Jaeran LEE, Sok Won KIM*

Department of Physics, University of Ulsan, Ulsan 44610, Korea
Correspondence to:
Received April 10, 2019; Revised July 12, 2019; Accepted July 12, 2019.
cc This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Can the motion of a particle motion in volume of a few femto-litres? It is well known that the observation of a particle is movement by using visible light is well to be impossible because the resolution of a confocal microscope is limited by the wavelength. Although observing the motion of a single particle is not possible, how each particle movers on average can be measured. Today's photometric techniques can estimate the size, the velocity, and the properties of particles by measuring the weak fluctuation of light emitted from nanometer-sized particles through some well-known physical laws. Here, we briefly introduce the basic principles of fluorescence correlation spectroscopy and its applications.
PACS numbers: 33.50.Dq, 42.62.Fi, 66.10.C$-$, 42.25.Ja
Keywords: Fluorescenc correlation spectroscopy, Rotational diffusion, Polarization

August 2019, 69 (8)
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