npsm 새물리 New Physics : Sae Mulli

pISSN 0374-4914 eISSN 2289-0041


Research Paper

New Phys.: Sae Mulli 2018; 68: 1147-1150

Published online October 31, 2018

Copyright © New Physics: Sae Mulli.

Analysis of the Temperature and the Impedance Characteristics in the Gelatin Methacryloyl Hydrogel Crosslinking Process

단백질 기반 수화젤의 광 경화 과정 임피던스 및 온도 특성 해석

Junhee PARK1, Taekyun YOO1, Jiseung LEE2, Chan Heum PARK2, Jae-Hyeon KO3, Moongyu JANG*4

1 Department of Nano-Medical Devices Engineering, Hallym University, Chuncheon 24252, Korea
2 Nano-Bio Regenerative Medical Institute, School of Medicine, Hallym University, Chuncheon 24252, Korea
3 Department of Physics, Hallym University, Chuncheon 24252, Korea
4 Department of Nano-Medical Devices Engineering and Department of Materials Science and Engineering, Hallym University, Chuncheon 24252, Korea


Received: July 30, 2018; Revised: August 20, 2018; Accepted: August 20, 2018

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.


In this study, the impedance and the temperature changes of gelatin methacryloyl, a protein based hydrogel used for various studies such as cell culture studies, were measured in real time. Sensors capable of simultaneously measuring the impedance and the temperature were fabricated using semiconductor and microelectromechanical systems (MEMS) processes. The occurrence of an endothermic reaction in the crosslinking of methacrylic anhydride, a crosslinking agent, was confirmed by the measurement of the temperature. Also, the measured impedance monotonically decreased during the gelatin methacryloyl hydrogel process.

Keywords: GelMA, Sensor, ECIS, Impedance, Temperature

본 연구에서는 세포 배양 연구 등 다양한 연구목적으로 사용되고 있는 단백질 기반 수화젤인 젤라틴 메타크릴로일(Gelatin Methacryloyl, GelMA)의 광 경화 과정에서 임피던스와 온도 변화를 실시간으로 측정하였다. 측정을 위해 임피던스와 온도를 동시에 측정할 수 있는 센서를 반도체 및 미세전자기계시스템 (microelectromechanical systems, MEMS) 공정을 사용하여 제작하였다. 온도 측정을 통해 광 경화 과정에서 가교제인 메타크릴릭 언하이드라이드(Methacrylic anhydride, MAA)의 가교 결합에서의 흡열반응을 확인하였다. 또한 고분자 형성 과정에서 젤라틴과 PBS용매, 고분자 사이에서 광 경화가 진행됨에 따라 전하 이동에 의해 임피던스가 감소하는 결과를 확인하였다.

Keywords: GelMA, 임피던스/온도 센서, 경화, 단백질 기반 수화젤

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