npsm 새물리 New Physics : Sae Mulli

pISSN 0374-4914 eISSN 2289-0041
Qrcode

Article

Research Paper

New Phys.: Sae Mulli 2019; 69: 846-854

Published online August 30, 2019 https://doi.org/10.3938/NPSM.69.846

Copyright © New Physics: Sae Mulli.

Review of the Principles and the Applications of Fluorescence Correlation Spectroscopy

Jaeran LEE, Sok Won KIM*

Department of Physics, University of Ulsan, Ulsan 44610, Korea

Correspondence to:sokkim@ulsan.ac.kr

Received: April 10, 2019; Revised: July 12, 2019; Accepted: July 12, 2019

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Can the motion of a particle motion in volume of a few femto-litres? It is well known that the observation of a particle is movement by using visible light is well to be impossible because the resolution of a confocal microscope is limited by the wavelength. Although observing the motion of a single particle is not possible, how each particle movers on average can be measured. Today's photometric techniques can estimate the size, the velocity, and the properties of particles by measuring the weak fluctuation of light emitted from nanometer-sized particles through some well-known physical laws. Here, we briefly introduce the basic principles of fluorescence correlation spectroscopy and its applications.

Keywords: Fluorescenc correlation spectroscopy, Rotational diffusion, Polarization

Stats or Metrics

Share this article on :

Related articles in NPSM