npsm 새물리 New Physics : Sae Mulli

pISSN 0374-4914 eISSN 2289-0041
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Research Paper

New Phys.: Sae Mulli 2020; 70: 1015-1020

Published online November 30, 2020 https://doi.org/10.3938/NPSM.70.1015

Copyright © New Physics: Sae Mulli.

Growth and drug Reaction Monitoring of NIH 3T3 Cells Using Impedance Biosensor

Gayoung LEE1, Jaehun JEONG1, Moongyu JANG1,2*, Sooyong SHIN3, Sungho JEON3

1School of Nano Convergence Technology, Hallym University, Chuncheon, 24252, Korea

2Center of Nano Convergence Technology, Hallym University, Chuncheon, 24252, Korea
3Department of Life Science and Multidisciplinary Institute, Hallym University, Chuncheon 24252, Korea

Correspondence to:jangmg@hallym.ac.kr

Received: August 31, 2020; Revised: October 5, 2020; Accepted: October 5, 2020

Abstract

An impedance biosensor to monitor cell growth and cell-drug reactions in real time was manufactured on slide glass by using a semiconductor process. The impedance pattern was deposited by using 80-nm-thick platinum for biocompatibility and a sputtering system. After the formation of a Pt electrode, a PDMS well was attached for the cell culture. After sterilization and coating the inside of the well with Poly-L-lysine, NIH 3T3 cells (-- 10K cells/well) were added to the wells and cultured for 43 hours. Impedance signals with the variations of frequency from 500 Hz to 150 KHz were continuously monitored for each 10 minutes during 43 hours. The capacitance showed a gradual increase with increasing number of NIH 3T3 cells. After a 43-hour growth, 10 $\upmu$g/mL puromycin (Gibco, A11138-03) was injected for the monitoring the cell-drug reaction. After the injection of puromycin, the capacitance was almost fixed for about 10 hours. After 10 hours, the capacitance rapidly decreased within the next 10 hours. In this study, we could monitor the state of cells and the cell-drug reactions by using a impedance biosensor chip developed using the electrical cell-substrate impedance sensing (ECIS) technique. The developed impedance biosensor could be applied in the wide area of bio-medicine, for example, non-destructive real-time cell growth and cell-drug reaction monitoring.

Keywords: Biosensor, Impedance, Real-time monitoring, Cell growth, Cell-drug reaction

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